Haynes Lab:Notebook/CRISPR Editing/2015/10/07

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10/07/2015

PCR purification

Cell type drug siRNA drug gRNA rep 260 280 260/280 ng/µL ul for 400ng ul ann buff ul water
Gal4-EEDdoxEZH2purog02510.1040.0571.834104.3993.831.509.67
Gal4-EEDdoxEZH2purog02520.0870.0461.87686.5284.621.508.88
Gal4-EEDdoxEZH2purog02530.0960.0521.82195.5674.191.509.31
Gal4-EEDdoxnonepurog02510.1170.0631.863116.9363.421.5010.08
Gal4-EEDdoxnonepurog02520.0770.0431.81377.3145.171.508.33
Gal4-EEDdoxnonepurog02530.0880.0461.90188.0684.541.508.96
Cell type drug siRNA drug gRNA rep 260 280 260/280 ng/µL ul for 400ng ul ann buff ul water
Gal4-EEDdoxEZH2purog03410.0540.0311.75554.1267.391.506.11
Gal4-EEDdoxEZH2purog03420.0630.0351.82963.1316.341.507.16
Gal4-EEDdoxEZH2purog03430.0540.0321.70654.2677.371.506.13
Gal4-EEDdoxnonepurog03410.0680.0381.8168.0145.881.507.62
Gal4-EEDdoxnonepurog03420.0520.0291.81152.0327.691.505.81
Gal4-EEDdoxnonepurog03430.050.0281.77750.2737.961.505.54

g034 EcoRI donor

260 280 260/280 ng/µL
0.1320.0711.864132.19


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