Haynes Lab:Notebook/CRISPR Editing/2015/10/11

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10/11/2015 Surveyor of g055 and test of g054, 42deg for 1h, 1.9ul stop solution, dilute 1:20, run on BA Set up phusion PCR of sheared Luc14 gDNA with P160/238 P161/233 P237/239 annealing temp 60, ext time 2min changed media on transfected cells to media with antibiotics (including dox or puro)