Haynes Lab:Notebook/CRISPR Editing/2016/05/21

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05/21/2015

Purified nested PCR for Luc14 g025 and g048. Stacked the previously purified g048 DNA

Cell type gRNA rep 260 280 260/280 ng/┬ÁL ul for 400ng ul ann buff ul H20
Luc14g02510.0350.021.79235.27911.338189861.52.161810142
Luc14g02520.020.0111.86419.71320.291178411.5-6.79117841
Luc14g02530.0190.011.89719.23720.793262981.5-7.293262983
Luc14g04810.0280.0161.79827.86714.353895291.5-0.853895288
Luc14g04820.0340.0191.81333.85711.814395841.51.685604159
Luc14g04830.0380.0211.77938.05710.510549961.52.989450035

Not enough g025. Will start doing 100ul reactions since I reduced the number of cycles.



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