Haynes Lab:Notebook/CRISPR Editing/2016/06/02
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06/02/2016Ran g055 nested PCR again, used 05ul of phusion from the wang lab, 32 cycles. ran on gel, bright bands, no band in neg ctrl lane. column purified, set up annealing reaction
Plate 6
possible plate 7
Primer set 3 master mix
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