Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/09/08

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Redid PCR of all the reactions that didn't produce the desired product (see yesterday's chart) a lower annealing temp (50C)

NumberTemplatePrimer 1Primer 2Expected sizeWorked?
1I13522 3P060P0623136 No
2K084007 12.9P069P070645 Yes
3K084007 18.3P069P070645 No
4K092900-E0420 80.5P077P078765Probably not
5Part B1P081P082980 No
6Part B2P081P082980No
7Part B3P081P082980No

GG PCR round 2, not the ugliest gel I've run

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