Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/09/28

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mm/dd/yyyy 8am Sender/Receiver OD650 GFP RFP EsaI 0.679 1500 0.671 1545 0.634 1402 RhlI 0.755 13497 0.792 14323 0.763 13992 Ctrl 0.736 6645 0.712 6485 0.740 6598 LuxR-GFP 0.548 1619 0.563 1858 0.576 1957 LuxR-RFP 0.576 768 0.564 682 0.562 694 Combined 3-5mL cultures of each sender into 15mL conicals. Spun in Wang lab large centrifuge spun receiver cells in 2mL tubes at 12k g for 5min I didn't write this down but I'm assuming I split the receiver cells into the sender supernatant in singlet.