Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/10/21

Project name

Main project page Previous entry      Next entry

mm/dd/yyyy Retry ligation from 10/19/13 again with gel extracted J06702 Backbone: J06702 E/X: 44 ng/ul Cut, gel purified Insert: Gblck pLac-RBS E/S: 2.6ng/ul Cut, column purified same calculations from before, still only actually tried 3:1 and bb ctrl 1:1 2:1 3:1 Bb ctrl insert 0.5 1.0 1.5 0 vector 1.13 1.13 1.13 1.13 2x Roche ligation buffer 5 5 5 5 T4 ligase 1 1 1 1 H20 2.37 1.87 1.37 2.87 ligations ran for 3 hours at RT Incubate with 50ul DH5αT for 45 min on ice heat shock for 30s at 42°C ice for 2min spread

|}