Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/02/26

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02/26/2014

Spun overnight cultures of GFP-Senders at 13.1g for 10 minutes in 2mL tubes in benchtop centrifuge. Filtered using Fuqing's 0.45μm sterile filters and syringes. Made 5mL of 1% LuxI media. 5mL of LB+amp and 50ul LuxI sender.
Set up microfluidics with David Menn. LuxI inducing LuxR-GFP at 1%.
Made dilutions of filtered sender media. Wanted to make sure each concentration has the same ratio of spent media to fresh media. Made 1800ul. 300ul for 3 replicates of each dilution. 1500ul for flow cytometry. Can take some off the top at multiple time points.

%media Total media ul sender ul LB media ul spent ctrl media
10180018016200
5180090162090
2.51800451620135
1.25180022.51620157.5
0.625180011.251620168.75
0.312518005.6251620174.375


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