Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/02/26
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Spun overnight cultures of GFP-Senders at 13.1g for 10 minutes in 2mL tubes in benchtop centrifuge. Filtered using Fuqing's 0.45μm sterile filters and syringes. Made 5mL of 1% LuxI media. 5mL of LB+amp and 50ul LuxI sender.