Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/15

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mm/dd/yyyy Receiver golden gate with newly prepped receiver intermediate vector Part ng/µL fmol/ul ml for 200fmol Receiver int vec 56.995 27 7.407407407 Reaction Rec Bb pLux-GFP pLac-LuxR BsmBI T4 Ligase T4 Ligase buffer H2O Bb ctrl 7.4 0 0 0.5 1.25 2 8.85 Lux Rec 7.4 2.94 2.25 0.5 1.25 2 3.66 Rhl Rec 7.4 2.78 1.68 0.5 1.25 2 4.39 Esa Rec 7.4 4.35 2.7 0.5 1.25 2 1.8