Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/28

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Set up golden gate reactions for RhlR receiver with different concentrations of backbone to try to get as close to 0 colonies on the backbone control plates

Label Bb concentration Bb ul RhlR ul pRhl ul BsmBI T4 Ligase T4 Ligase buffer H2O
120 fmol0.741.682.780.51.2521.05
210 fmol0.371.682.780.51.2521.42
32 fmol0.74 of 1:10 dil1.682.780.51.2521.79
420 fmol0.74000.51.2525.51
510 fmol0.37000.51.2525.88
62 fmol0.74 of 1:10 dil000.51.2526.25

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