Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/02/03

From OpenWetWare

Jump to: navigation, search
Project name Main project page
Previous entry      Next entry

02/03/2015

I added enzymes to my insert sample (which was PCR-purified the day before) for digestion. I first added water to a 200μL PCR tube, then I added the sample, buffer, and two enzymes.


Restriction digest

Thing vol in ul
1ug sample9.17
H2O6.83
10x buffer2
PstI1
BbsI1
Total20


Purified with PCR clean up kit

Sample Read# 260 280 260/280 ng/µL
insert for ryan0.0120.0071.6212.1





Personal tools