Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/02/03

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02/03/2015 I added enzymes to my insert sample (which was PCR-purified the day before) for digestion. I first added water to a 200μL PCR tube, then I added the sample, buffer, and two enzymes. Restriction digest Thing vol in ul 1ug sample 9.17 H2O 6.83 10x buffer 2 PstI 1 BbsI 1 Total 20 Purified with PCR clean up kit Sample Read# 260 280 260/280 ng/µL insert for ryan 0.012 0.007 1.62 12.1