I cut some of the PCR product I created on 2/5/2015 with PstI and BbsI again (see protocol on 2/6/2015).
This time, I completed the protocol 4 times in 4 separate tubes. Then, I PCR purified the 4 tubes all in the same column. (20μL in each tube = 80μL total for PCR purification. During the elution step, I eluted my column with 20μL of elution solution instead of 50μL to increase my final concentration. After the first round of elution, I kept the column and applied another 20μL of elution solution to it, and eluted again. The first elution is expected to have a significantly higher concentration than the second.
PCR Purification results
| 1 (first elution)||0.062||0.036||1.746||62.034
| 2 (second elution)||0.013||0.01||1.391||13.462