Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/02/23

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02/23/2015

Today I prepared the liquid colonies that I made on Friday for sequencing. I transferred the liquid to 2mL tubes and spun the tubes, poured out the LB broth and isolated the pellet of cells. Then I mini prepped them. The following shows the concentrations of each tube after the mini-prep.

Sample Read# 260 280 260/280 ng/┬ÁL
60.060.0331.83260.226
80.060.0331.78959.697
90.0580.0321.79257.778
100.0590.0331.78559.318


Rene sent the samples out for sequencing later in the day.




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