Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/03/25

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PCR Purification of a double digest reaction of my ligation created 3-19-2015. I used EcoR1 and XBaI
This will be the "new backbone" used to insert synthase genes in the future.

Sample Read# 260 280 260/280 ng/┬ÁL

I PCR-purified to get rid of the insert because Ryan designed the insert to be small enough that it will be washed away during the purification. As a result, our product should consist of just the backbone.

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