Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/03/25

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03/25/2015 PCR Purification of a double digest reaction of my ligation created 3-19-2015. I used EcoR1 and XBaI This will be the "new backbone" used to insert synthase genes in the future. Sample Read# 260 280 260/280 ng/µL 1 0.01 0.007 1.516 9.897 I PCR-purified to get rid of the insert because Ryan designed the insert to be small enough that it will be washed away during the purification. As a result, our product should consist of just the backbone.