Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/05/01
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PCR Optimization of RhlI. 4 PCR tubes with DMSO, 4 PCR tubes without DMSO. Tubes were placed in the PCR machine with a temperature gradient. (range?)
PCR-purified synthase PCR products. Next, they are ready to be digested and then ligated with the backbone.