Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/05/01

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05/1/2015 PCR Optimization of RhlI. 4 PCR tubes with DMSO, 4 PCR tubes without DMSO. Tubes were placed in the PCR machine with a temperature gradient. (range?) PCR-purified synthase PCR products. Next, they are ready to be digested and then ligated with the backbone. Sample Read# 260 280 260/280 ng/µL Aub 0.025 0.014 1.814 24.637 Bja 0.033 0.017 1.938 33.411 Bra 0.045 0.025 1.782 44.594 Cer 0.033 0.018 1.852 33.122 Rpa 0.049 0.028 1.736 48.839 Esa 0.037 0.02 1.893 37.255 Lux 0.045 0.023 1.986 45.285 Las 0.033 0.019 1.802 33.354 Sin 0.024 0.013 1.8 23.961