Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/05/01

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05/1/2015

PCR Optimization of RhlI. 4 PCR tubes with DMSO, 4 PCR tubes without DMSO. Tubes were placed in the PCR machine with a temperature gradient. (range?)

PCR-purified synthase PCR products. Next, they are ready to be digested and then ligated with the backbone.

Sample Read# 260 280 260/280 ng/ยตL
Aub0.0250.0141.81424.637
Bja0.0330.0171.93833.411
Bra0.0450.0251.78244.594
Cer0.0330.0181.85233.122
Rpa0.0490.0281.73648.839
Esa0.0370.021.89337.255
Lux0.0450.0231.98645.285
Las0.0330.0191.80233.354
Sin0.0240.0131.823.961

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