Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/05/18
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I ran the PCR-optimization samples from last Friday on a gel; 4 were treated with DMSo and 4 were treated without (used water in place of it). The ones than were ran without DMSO appeared to have darker bands and lighter off-bands, so we decided to PCR purify those four.
We also fast-transformed AubI and BjaI from last Monday's ligations with Ryan's new cells (NEB 10β), because we have been seeing colonies on the negative control using René's cells. In addition to the two ligations, we made a negative control plate and a backbone-only control plate with the double digested EX sender backbone.