Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/06/17
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Two bands for CerI were expected since there are two EcoRI sites in the synthase. EsaI 2 and 3 looked like they were successful; these bands are longer than the others—since part of EsaI appeared to be truncated last time, the shorter bands probably represent the truncated plasmid.