Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2016/05/11
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Yesterday, I picked 10 colonies from the pLas [1/2 plate]. Today I miniprepped 8 of them. Two out of the 10 liquid cultures did not grow. (Media was clear and no cells formed at the bottom of the tubes after I centrifuged them). Concentrations were in the 300ng/uL range (except for LasR 9—17ng/uL).
Next step is to digest verify plasmids with EcoRI and KpnI.
Next, I ran a gel with these digests. Results pending.