UPLassay
- This is a test run for RT-PCR training. Using gene targets identified in prior research to pancreas differentiation gene sequences.
Reaction List
REACTION LIST
|
Template cDNA |
Gene Target
|
Rxn 1: |
treated cells |
PDX1
|
Rxn 2: |
treated cells |
MAFA
|
Rxn 3: |
treated cells |
GLP1R
|
Rxn 4: |
treated cells |
PCSK1
|
Rxn 5: |
treated cells |
IAPP
|
Rxn 6: |
treated cells |
KCNQ2
|
Rxn 7: |
treated cells |
GAPD (reference gene)
|
Rxn 8: |
untreated cells |
PDX1
|
Rxn 9: |
untreated cells |
MAFA
|
Rxn 10: |
untreated cells |
GLP1R
|
Rxn 11: |
untreated cells |
PCSK1
|
Rxn 12: |
untreated cells |
IAPP
|
Rxn 13: |
untreated cells |
KCNQ2
|
Rxn 14: |
untreated cells |
GAPD (reference gene)
|
Rxn 15: |
no template |
PDX1
|
Rxn 16: |
no template |
MAFA
|
Rxn 17: |
no template |
GLP1R
|
Rxn 18: |
no template |
PCSK1
|
Rxn 19: |
no template |
IAPP
|
Rxn 20: |
no template |
KCN2Q
|
Rxn 21: |
no template |
GAPD (reference gene)
|
- Run 3 Each
- 45 Reactions Run
- Using Variation 2
Variation 2
- Using as Reference, discount gene names on left side
Primers
PRIMERS LIST
|
Roche Name |
Left Primer |
Right Primer |
UPL probe
|
PDX1 |
NM_000209.3 |
aagctcacgcgtggaaag |
gccgtgagatgtacttgttgaa |
#78, cat.no. 04689011001
|
MAFA |
NM_201589.3 |
agcgagaagtgccaactcc |
ttgtacaggtcccgctcttt |
#39, cat.no. 04687973001
|
GLP1R |
NM_002062.3 |
gtggcggccaattactactg |
ggccagcagtgtgtacagg |
#22, cat.no. 04686969001
|
PCSK1 |
NM_000439.4 |
caagagcttgtgaaggacaaga |
tctttcagccaagagcacag |
#1, cat.no. 04684974001
|
IAPP |
NM_000415.2 |
ttaccaaattgtagaggctttcg |
ccctgcctctatacactcactacc |
#77, cat.no. 04689003001
|
KCNQ2(4) |
NM_172108.3 |
gacgtcatcgagcagtactca |
cccacgatctggtccact |
#56, cat.no. 04688538001
|
GAPD (Ref) |
NM_002046.3 |
agccacatcgctcagacac |
gcccaatacgaccaaatcc |
#60, cat.no. 04688589001
|
|
|
|