Haynes Lab:Notebook/David Barclay Undergrad Training/Plasmid Transformation/2015/07/15

Haynes BioBrick Cloning	Main project page Previous entry      Next entry
Continuation of Restriction Digests and Gel Electrophoresis Restriction Digest Table Checked plasmid minipreps with EcoRI/PstI digests Reagent Volume Expected: 1. hPCD = 2000 bp, 4500 bp 15 μL/lane; 1% agarose; Ladder DNA(plasmid) 5.0 μL 10X FD Buffer 1.5 EcoRI 1.0 PstI 1.0 dH2O 6.5   15 μL --> 37°C/ 15 min. Conclusion