Haynes Lab:Notebook/David Barclay Undergrad Training/Plasmid Transformation/2015/08/04

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Plasmid Extraction and Validation

  • BD004 MV2
  • BD006 MV2
  • Culture inoculation 8-4-15 (19 hour incubation)
    • Culture 1-4: growth
    • Culture 2-4: growth
    • Culture 1-6: growth
    • Culture 2-6: growth
  • B0004 MV2
    • Streak plate Colony 1: growth
    • Streak plate colony 2: growth
  • BD006 MV2
    • Streak plate colony 1: growth
    • Streak plate colony 2: growth
  • Sample 1-4= B0004 MV2 colony 1
    • OD260/280=1.90
    • Concentration: 229.7 ng/μL
  • Sample 2-4 = B0004 MV2 colony 2
    • OD260/280=1.99
    • Concentration: 125.05 ng/μL
  • Sample 1-6 = B0006 MV2 colony 1
    • OD260/280= 1.957
    • Concentration: 160.15 ng/μL
  • Sample 2-6= B0006 MV2 colony 2
    • OD260/280 = 2.103
    • Concentration: 35.733 ng/μL
  1. used 50 μL H2O for DNA elution


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