Haynes Lab:Notebook/Engineering PC-TFs/2012/01/26

Transformation

1. hPCD 2. flyPCD 3. fshPCD 4. no DNA

• Warm 4 Amp agar plates (100 µg/mL) @ 37°C
• 0.5 µL DNA + 10 µL dH2O (in 0.5 mL tubes)
• Add 30 µL DH5 Turbo compotent cells to DNA + dH2O
• Put immediately on ice --> incubate on ice for 5 min.
• Label plates
• Pipette cells + DNA onto agar --> Pour 10-15 beads onto the cells on the agar.
• Shake the plates to spread the cells.
• Invert. Dump dirty beads.
• Incubate plates @ 37°C for 6-8 hours.