Haynes Lab:Notebook/Engineering PC-TFs/2015/05/05

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Summary

  • Retransformed relevant parts.

Parts:
BBa_J176040 KAH021 60 PLflex, (GGGGS)4 peptide linker, flexible
BBa_J176130 KAH022 240 PLflex4, 4x peptide linker, flexible; (GGGGS)16
BBa_J176131 KAH023 60 PLrigid, (EAAAR)4 peptide linker, rigid; alpha helix secondary structure; see Yan et al. 2007
BBa_J176132 KAH024 240 PLrigid4, 4x peptide linker, rigid; (EAAAR)16; alpha helix secondary structure; see Yan et al. 2007
BBa_S04707 KAH90 1053 fshPCD : [mCh : VP64] DNA

Used 1μL plasmid DNA (concentration ~200ng/μL), 10μL dH2O, and 50μL DH5α-Turbo cells. Incubated mixture for 5 minutes on ice and then plated on warmed labeled Amp plates. Water and cells served as the negative control.


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