Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR

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Project Description/Abstract

The Gal4EED/luc cell line is being used to measure large-scale changes in chromatin structure using the luciferase assay. This assay has poor resolution at the cellular scale and is difficult to perform; therefore, I have been tasked with editing out the luciferase gene and replacing it with a cyan fluorescent protein (CFP) as a more suitable marker for chromatin modification.

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