Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/03/24
|DBN002 Gel Extraction & PCR|| Main project page|
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I cut out the 1500bp band in lane 8 from the agarose gel run yesterday and purified the DNA using the Sigma GenElute Gel Extraction Kit (protocol can be viewed here). After extracting the DNA, I performed a thermal gradient PCR on the fragment using the following quantities of reagents:
Thermal cycler program:
Thermal gradient is from 59°C to 66°C. Program name is DBN20150324.