Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/05/05
|Plasmid Verification (multiple)|| Main project page|
Previous entry Next entry
All six liquid cultures grew as expected. Harvested cells and performed a plasmid miniprep for each. The BioBrick cloning constructs ( from KAH57 & KAH104) I'm naming DBN006.
Will verify via restriction digest tomorrow.
Restriction Digest (05/06)
Incubate at 37°C for 10 minutes, then run total volume on the gel.
Colonies 1 and 3 contain inserts of the expected size.