Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/10/28
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Phusion PCR of parts for LCR | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OverviewWe're using the Phusion polymerase to create excess template in anticipation of performing ligase-cycling reaction (LCR) in the near future. This will allow us to incorporate a zeocin resistance (ZeoR) gene upstream of the HPK-CFP fluorescent reporter. MethodsReaction volume: 100 µL (split into 4 tubes to be pooled later)
Reactions:
All primers have annealing temperature at 60°C Thermal cycler program:
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