Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/11/30
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Performing LCR on the PNK-treated parts from last week.
Thermal cycler program:
Follow the instructions in the protocol described here. Setting up three plates:
May have to perform a PCR clean-up on the sample next time before transforming cells.
Colony PCR Results
Performed colony PCR on the one colony on the sample plate. No product. Giving up on LCR and switching to Golden Gate assembly.