Haynes Lab:Notebook/Jan/2014/09/18

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Extraction of KAH126

  • Name: KAH126
    • Date of culture inoculation: 9/17/14
    • Results for Cultures 1 and 2: Mixture is cloudy so growth in both. Success.
    • Results for Streaks 1 and 2: Growth in both areas along zig-zag line, although the growth is splashed across the dividing line. Overall success.
  • Sample 1: KAH126 colony 1;
  • Sample 2: KAH126 colony 2;
  • Sample 3: KAH126 (control DNA);


DNA Concentration Data

Plasmid OD260 OD260/280 ng/μL
1. Plasmid 1
2. Plasmid 2


Restriction Digest Table

  • Checked plasmid minipreps with EcoRI/PstI digests
Reagent Volume Expected:
1.
2.

15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 8.5
  15 μL --> 37°C/ 15 min.


DNA Sequencing Samples

  • Submitted to DNASU on:
  • Order Number:



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