Haynes Lab:Notebook/Jan/2015/04/07

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04/07/15 Minipreps - BD004 assembly Minipreps - BD004 assembly ---Karmella 17:57, 7 April 2015 (EDT): Sigma GenElute, minipreps from 2x 5 mL cultures Elute & back-elute w/ 70 μL elution sln. BD004 a BD004 b Digests (Fermentas FD) E/S to prep for insertion into MV2 (E/X) Reagent Volume Expected: 1,2. BD004 (E/S) = 4096*, 3200 *Bands cut & purified 30 μL/lane, 1% agarose; Ladder DNA (plasmid) 20.0 μL 10x buffer 3.0 enzyme 1 1.0 enzyme 2 1.0 dH2O 5.0   30 μL --> 37°C/ ~30 min. Conclusions tba Miniprep conc.'s Sample OD260 260/280 ng/μL 1. BD004 a mp 0.357 1.92 356.67 2. BD004 b mp 0.262 1.89 262.29 Gel purification conc.'s Sigma gel extraction kit, eluted & back-eluted w/ 30 μL elution sln Sample OD260 260/280 ng/μL 1. BD004 a (E/S) 0.032 1.73 32.13 2. BD004 b (E/S) 0.02 1.62 19.79