PCR and Agarose Gel Electrophoresis on 4-5, 4-7, 4-9, and 6-4
- Round 2: Genomic DNA prepped from candidate clonal lines
- To be done after chosing a single primer pair per validation region
- BD4-5, Chromometer 2
- BD4-7, Chromometer 2
- BD4-9, Chromometer 2
- BD6-4, Chromometer 1
ROUND 2 PCR: Candidate clonal lines
| Reagent
|
Vol
|
Mix* (x #)
|
Expected:
1. CMV = 588
|
Hover name
5 μL/lane; 1% agarose; Ladder
|
| genomic OR plasmid DNA |
1.0 / 0.5 |
7.0 / 4.5
|
| 10 μM forward primer |
1.0 |
---
|
| 10 μM reverse primer |
1.0 |
---
|
| 2x GoTaq green |
10.0 |
70.0
|
| dH2O |
7.0 / 7.5 |
49.0 / 52.5
|
| |
20.0 μL |
---
|
Note: *Prep one Mix per template
- For each template, aliquot 18.0 master mix into 6 tubes
Thermal cycler: Labnet - GOTAQ
- 95°C 3 min.
- 30x[95°C, 30 sec; 57°C 30 sec; 72°C 30 sec]
- 72°C 3 min.
- 4°C, ∞
|
Project name
Main project page
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