Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/02/05

RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines

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Summary SKNSH cells in 6-well plate are ready for transfection, so today I'll be doing that (again). Also preparing more U2OS medium with 10% FBS and 1% pen/strep, and passaging KAH126/U2OS and SKNSH cultures. Goals Make more U2OS medium (McCoy's 5A w/ 10% FBS, 1% P/S) Transfect SKNSH cells in 6-well plates Passage KAH126/U2OS Passage SKNSH Protocol passaging cells transfection Notes Observations, measurements, placeholder info etc. go here. Results/Conclusions Data collected from your experiments, significance of findings, future steps etc. all go here. Like the introduction of the page, this should also be in paragraph format and summarize your findings so a new reader can follow along.