Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/06/16

RNA Quantification

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Overview Checked concentration of RNA from RNeasy preps performed last week. Expected 260/280 ratio for pure RNA is ~2.0. Sample ID Conc. (ng/µL) St Dev (ng/µL) 260/280 K562 pMaxGFP 24hr 638.0 1.72 2.09 K562 KAH126-MV2 24hr 1 609.4 14.42 2.07 K562 KAH126-MV2 24hr 2 670.69 10.5 2.09 K562 pMaxGFP 48hr 823.5 13.52 2.08 K562 KAH126-MV2 48hr 1 970.7 7.27 2.09 K562 KAH126-MV2 48hr 2 835.4 14.52 2.09 K562 no plasmid 24hr 969.2 10.00 2.12 K562 no plasmid 48hr 802.2 12.68 2.10 DNASU needs 50-100 ng of RNA in 5 µL total volume (10-20 ng/µL conc.). So I'll need to make some dilutions first before submitting anything to them.