Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/01/26
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Still checking on the cultures, going to harvest the ones induced to create PcTF-RFP later in the week if their expression levels look good. Also submitting the RNA harvested last week to DNASU, where they'll create cDNA and then quantify it using real-time PCR.
Observations, measurements, placeholder info etc. go here.
"Negative control" is a sample not treated with doxycycline but still containing RNA.
Samples have been submitted to DNASU for creation of cDNA followed by RT-PCR.
Fluorescence microscopy images of KAH126-U2OS cells after 4 days of induction:
Fluorescence microscopy images of U2OS cells 4 days after transfection: