Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/05/11

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Fluorescence microscopy images of transfection results Main project page
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Overview

U-2 OS culture was transfected on Friday, May 8. I'm now checking on the success of those transfections.

There were three transfections performed (in duplicate): KAH187-MV8 (positive control, CFP); KAH126-MV2 (RFP); and KAH87-MV10 (RFP).

Images

KAH187-MV8


KAH126-MV2

KAH87-MV10

Results

Positive control worked as expected. Surprisingly, KAH126 did as well; might be due to low cell density prior to transfection (~70% confluency). KAH87-MV10 did not show any transfection efficiency - this is most likely due to the fact that the insert in the plasmid was placed backwards. Will repeat KAH87-MV10 once I have a better copy.



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