Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/05/15

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Transfection of K562 using KAH187-MV8, KAH126-MV2, KAH87-MV10 Main project page
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Overview

Repeating last week's experiment with K562 and a working copy of the KAH87-MV10 plasmid.

Transfection Setup

See the Haynes Lab transfection protocol for details.

One difference: final volume of antibiotic-free medium in each well is 2mL, not 4mL. All other values kept the same.

Plasmid Conc (ng/µL) Vol DNA (µL) Vol water (µL)
KAH187-MV815181.318.7
KAH126-MV28092.517.5
KAH87-MV10350.35.714.3
Reagent Volume (µL)
DNA20
Opti-MEM570
PLUS reagent2.5
Lipofectamine LTX7.5

Cell density as measured by hemacytometer is 460,000 cells per well.



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