Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/06/02
|DNA concentration of KAH126-MV2|| Main project page|
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I currently have a large stock of KAH126-MV2 at 809 ng/µL. I want to concentrate it further to about 2400 ng/µL. Will follow the procedure given in the GenElute HP Endotoxin-Free Plasmid Maxiprep Kit.
Starting volume of plasmid is 300 µL, final volume is 100 µL.
Final concentration is between 1660 ng/µL and 2257 ng/µL. I'm going to guess it's about 2000 ng/µL.