Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/06/12

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Overview

Collecting the first timepoint for the no plasmid control. Will collect the post-48hr sample tomorrow.

Microscopy

Well 1: no plasmid control, post-24 hrs

Well 4: no plasmid control, post-48 hrs

Flow Cytometry

Plot 1 (FSC-A/SSC-A)Plot 4 (FL3-A)Plot 6 (FL1-A)
AllCellsM1M2
CountVolume (µL)Events / µLCountEvents / µL% of AllCount% of This PlotCount% of This Plot
A01 Blank1411014000.00%0100.00%0100.00%
A02 1 no plasmid15,55210155511,704117075.26%370.32%170.15%
A03 4 no plasmid17,21810172213,026130375.65%300.23%150.12%

RNA Miniprep

Harvested well 1 and treated with Trizol, storing at -80 °C until more Qiagen RNeasy kits arrive.



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