Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2016/07/27
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1. U2OS-PcTF and U2OS-TF stable cell lines ChIP-PCR on (1) PcTF, (2) H3K27me3, (3) H3K4me3, and (4) PolII before, during, and after PcTF expression at endogenous enhancer(s)
Update: René is culturing cells!
Update: RNeasy kit ordered. Cells are still growing, looking good so far. Will harvest on Thursday, 7/28.
Update: Plasmid midiprep worked. Concentration is ~400 ng/µL, good 260/280 ratio. Verification image:
SK-N-SH and K562 cultures expanded growing in PS-β. U-2 OS split into 6-well plates.
Still need to check probes.
Update: RNA extraction successful. 260/280 values all close to 2.05. Sample concentrations:
Will pick 2 from each sample type to submit for RNA-Seq to DNASU.