Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2016/07/28
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1. U2OS-PcTF and U2OS-TF stable cell lines ChIP-PCR on (1) PcTF, (2) H3K27me3, (3) H3K4me3, and (4) PolII before, during, and after PcTF expression at endogenous enhancer(s)
Update: Harvested all 24 samples. Measured red fluorescence by flow cytometry before dissolving in TRIzol. Flow data:
Update: Transfected U-2 OS plate. Will begin taking photos, collecting flow data, and harvesting via TRIzol beginning tomorrow.
SK-N-SH and K562 cultures growing in PS-β.
Still need to check probes.
Will submit samples to DNASU tomorrow.