Haynes Lab:Notebook/Short Projects/2014/09/16

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09/16/14 - Jonah Thomas

  • Transformation - KAH60/pcVN into DH5α-turbo



Transformation

  • HEK cells did not express mCherry after transfection
    • Discarded all samples
  • Therefore, we will try to obtain higher quality plasmid by transforming the stock into bacteria
  • After transformation & growth, we will do a miniprep to get fresh plasmid DNA
    • To check quality of DNA, we will do a restriction digest

Transformation samples

  1. KAH60/VNpc (from Harvard stocks) - 0.5 μL in 10 μL dH2O
  2. 10 μL dH2O
  • Will check for colonies tomorrow morning



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