Haynes Lab:Notebook/Short Projects/2015/02/23

Haynes Lab - Short Projects

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02/23/15 - Alexander Ellingson PCR-verify activation domain primers Try different cDNA libraries for the #name# fragment to account for any mutations or deletions U2OS C002, 1:1000 cDNA dilution; #name# = #size#: #f-primer#/ #r-primer# SKNSH C001, 1:1000 cDNA dilution; same K562 C001, 1:1000 cDNA dilution; same Reagent Rxn1 Rxn2 Rxn3 Expected: 1. U2OS #name# = #size# 2. SKNSH #name# = #size# 2. K562 #name# = #size# Hover name 10 μL/lane, 1% agarose; Ladder Template 1.0 1.0 1.0 10 uM fwd primer 1.0 1.0 1.0 10 uM rev primer 1.0 1.0 1.0 2x GoTaq green 12.5 12.5 12.5 dH2O 9.5 9.5 9.5   25.0 25.0 25.0 Program: GOTAQ35cyc 95°C, 3 min 35x[95°C, 1 min; 57°C, 1 min; 72°C, 3 min] 72°C, 3 min 4°C ∞