Haynes Lab:Notebook/Short Projects/2015/06/11
|Haynes Lab - Short Projects|| Main project page|
Ben Nyer - 11/06/15
Cloning gRNAs into pSPgRNA for dCas9-HT binding to luc gene
Sequencing results came back for g031_pSPgRNA plasmid. Colony 1 contains errors in the insert region, but colony 2 is a match. All four gRNAs are in pSPgRNA and stored in 'Karmella Box 2' in the -20 °C freezer.