QiaPrep Spin
- Transfer 2mL of culture to round small round bottom tubes
- Divide plate and label (this will be your back up culture)
- Drag tip from tube lightly across the agar. Put the tip back in the tube. (Use forceps)
- Centrifuge for 3 min (make sure to balance and keep centrifuge at top speed)
- Dump waste from tubes
- Resuspend pellet in 250uL of PI buffer then vortex
- Add 250uL of P2 buffer and invert 6x
- Add 350uL of N3 buffer and invert 10x
- Centrifuge for 10 min
- Label column collection tubes (blue) and dump you centrifuged liquid in columns
- Spin for 1 min and dump waste
- Add 750uL of PE buffer
- Centrifuge for 1 min, dump, centrifuge for 1 min
- Place liquid in 1.5mL microcentrifuge tube and add 75uL of EB buffer directly above the white filter
- Centrifuge for 2 min toss blue column
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