Haynes Lab:Notebook/Synthetic Chromatin for Cancer Research/2015/10/28
Synthetic Chromatin for Cancer Research
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Miniprep pet28 vector
Gel verification of pet28. Image in dropbox.
PCR DT017, DT028, DT040 so we can put it into the pet28 vector (they are currently in V0120).
PCR clean up for DT017, DT028, DT040, and pet28.
Protocol for PCR clean up:
Add 5x volume PB
Add 1/10 PCR volume of 3M NaAC
Bind to column (1 min @ max speed, dump)
750ul PE (dump. 1 min @ max)
1 min spin
Elute with 35ul of H20
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