Haynes Lab:Notebook/TB Biosensor/2012/05/24

From OpenWetWare

Jump to: navigation, search
Project name Main project page
Next entry

Entry title

The following parts are Amp resistant and from the bacteria, Pantoea ananatis. The numbers correspond to the labeling used in the lab:


1. K118014; RBS + crtE; 2012 kit plate 2 well 18H http://partsregistry.org/Part:BBa_K118014)

2. K274100; RBS + crtE + RBS + crtI + RBS + crtB; 2012 kit plate 3 well 6H http://partsregistry.org/Part:BBa_K274100

3. K274200; RBS + crtE + RBS + crtB + RBS + crtI +RBS + crtY; 2012 kit plate 3 well 6L http://partsregistry.org/Part:BBa_K274200

4. K118002; crtB; 2012 kit plate 2 well 16F http://partsregistry.org/Part:BBa_K118002

5. K118006; RBS + crtB; 2012 kit plate 2 well 16N http://partsregistry.org/Part:BBa_K118006

6. I742161; RBS + crtI + RBS + crtB; 2012 kit plate 2 well 20C http://partsregistry.org/Part:BBa_I742161

7. K118003; crtI; 2012 kit plate 2 well 16H http://partsregistry.org/Part:BBa_K118003

8. K118005; RBS + crtI; 2012 kit plate 2 well 16L http://partsregistry.org/Part:BBa_K118005


I pipetted 10ul of water into each well in the kit plates and transfered the DNA to 1.5ml tubes. I added 30ul of thawed DH5alpha competent cells to each tube, gently mixed, and left the tubes on ice for 5 minutes. I then pipetted the cells onto LB+amp plates and left overnight at 37deg.


Personal tools