Making crt Fusion Proteins by Gibson Assembly
- Ernesto mixed the primers and templates below (to later PCR the overhangs onto the crt genes for Gibson assembly.)
Primer details with sequences
| Final Construct | Template | Forward Primer | Reverse Primer
|
| crtE - PLFlex - X | crtE | P001 | P010 |
|
| X- PLFlex - crtE | crtE | P015 | P002 |
|
| crtI - PLFlex - X | crtI | P005 | P012 |
|
| X- PLFlex - crtI | crtI | P017 | P006 |
|
| crtB - PLFlex - X | crtB | P007 | P014 |
|
| X- PLFlex - crtB | crtB | P019 | P008 |
|
| crtE - PLFlex - crtB | PLFlex | P009 | P020 |
|
| crtE - PLFlex - crtI | PLFlex | P009 | P018 |
|
| crtI - PLFlex - crtE | PLFlex | P011 | P016 |
|
| crtI - PLFlex - crtB | PLFlex | P011 | P020 |
|
| crtB - PLFlex - crtE | PLFlex | P013 | P016 |
|
| crtB - PLFlex - crtI | PLFlex | P013 | P018 |
|
|
Project name
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