Haynes Lab:Notebook:Shared/Cloning Bootcamp/2015/05/13

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Daniel Vargas - 05/13/15

mCherry pSB1A3 using Amp / DH5α-Turbo

Inoculation 04/28/15 http://openwetware.org/wiki/Haynes_Lab:Notebook:Shared/Cloning_Bootcamp/2015/04/28

Liquid culture 1: growth

Liquid culture 2: growth

Streak 1: growth

Streak 2: growth

Minipreps

Sample 1: mCherry pSB1A3 colony 1; 2H5Q; part 748bp; vector 2155

Sample 2: mCherry pSB1A3 colony 2; 2H5Q; part 748bp; vector 2155

DNA Concentration Data

Plasmid OD260 OD260/280 ng/μL
1. Plasmid 1 0.197 1.888 197.489
2. Plasmid 2 .130 1.889 129.815


Restriction Digest Table

  • Checked plasmid minipreps with EcoRI/PstI digests
Reagent Volume Expected:
1. Plasmid 1 = 746, 2155
2. Plasmid 2 = 746, 2155
Image:Http://openwetware.org/images/4/48/Screen Shot 2015-05-14 at 1.14.53 PM.png
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 8.5
  15 μL --> 37°C/ 15 min.







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