IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/28

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Monday 7/28

  • Started a seed of the seed of colony #3 from ligation plate #4.
  • Started a seed of the LacI promotor part (R0011) from Kylie

Set up a digestion of RBS+TesA to be run on a gel tomorrow

Reagent (μL) Volume (μL)
DNA(RBS+TesA plasmid) 10.0 μL
10X fast digest buffer 2.0
dH2O 6.0
XbaI 1.0
PstI 1.0
  20.0 μL --> 37°C/ ~overnight

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