IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/07/15

iGEM Project name 1

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Glycerol Stock Confirmation PCR Protocol #11 Samples: J23100, RBS YFP Terminator, pBad strong RBS RFP, Amp Construction Plasmid, chlor construction plasmid, control Gel Electrophoresis 1% gel with SYBR green all confirmed! Material 1 reaction Master mix 10x buffer 2.5uL 16.5uL Vf2 primer 1.25uL 8.25uL Vr primer 1.25uL 8.25uL dNTP 0.5uL 3.3uL sd water 19.3uL 127.38uL Taq Pol 0.2uL 1.32uL DNA 0uL 0uL Total 25uL 165uL